Regulatory

Part:BBa_K415511:Design

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)


pPAI2 L4R1 MammoBlock Entry Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 1082
    Illegal BglII site found at 1402
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 145
    Illegal SpeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1313
    Illegal BsaI site found at 1578
    Illegal BsaI.rc site found at 674
    Illegal SapI.rc site found at 1016


Design Notes

MIT iGEM 2010 Mammalian Part


Source

MIT iGEM 2010 Mammalian Part

References